| 摘要 |
Analyzing phenotype of HIV (human immune deficiency virus), resulting from one or more mutations in the viral genome that influence infection, in a patient sample, is new. Analyzing phenotype of HIV (human immune deficiency virus), resulting from one or more mutations in the viral genome that influence infection, in a patient sample, is new. Nucleic acids are extracted from the sample, segments of them amplified by PCR (polymerase chain reaction) using pairs of primers that flank a genomic sequence susceptible to mutation, and a first host cell (HC1) transfected with: (a) the amplicon; (b) a vector containing parts of the HIV genome required for replication, except for the amplified segment and optionally also the env gene; and (c) if the vector of (b) lacks the env gene, also a second vector containing this gene. Homologous recombination occurs to produce a chimeric virus and HC1 are cultured to produce viral particles (VP) during a single cycle of replication. VP are used to infect at least one second host cell (HC2) that contains a marker gene (MG) that is activated only after viral infection, then the expressed marker detected and/or quantified to detect at least one characteristic of the original HIV. An Independent claim is also included for a kit for performing the new process.
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