VIRAL EXPRESSION SYSTEM

摘要

The invention relates to viral expression systems comprising one first and one or more additional plasmid vectors containing two ITR sequences and the E4 gene and optionally, the E1 gene of the adenovirus, respectively, and a heterologous gene to be expressed. The vectors of the system differ from each other in terms of the presence of at least one of the aforementioned gene sequences. The E4 gene, the E1 gene and the heterologous gene are flanked by ITR sequences and are packed in eukaryotic cells in virions with the same after transfection. The invention also relates inter alia to methods for producing recombinant virions and for expressing a transgene in eukaryotic cells using said vector system and to virus stocks obtained according to said methods.