| 摘要 |
<p>A method of acquiring a gene specifically expressed in the head or nerve at the early development stage; and the gene specific to the nerve formation of a vertebrate which is obtained by this method. A cDNA library prepared from cells capable of inducing the head structure, which are obtained by treating a non-prospective germ layer piece of a Xenopus-origin blastula with 100 ng/ml of activin for 1 hour and then pre-incubating for 7 to 10 hours, is hybridized with [32P]-labeled cDNA which is prepared in the same manner from a gastrula at the stage before the formation of the nerve induction (stage 10). Unhybridized clones (negative clones) are selected and cDNAs of the obtained clones are subjected to the homology examination. By eliminating known genes, a gene specific to the nerve formation of a vertebrate, which is expressed in the head structure at the early development stage, can be obtained.</p> |
