摘要 |
<p>The present invention relates to a process for specifically modulating the properties of an intracellular target molecule T, and / or of a cellular component C which interacts directly or indirectly in a cell with T, said process comprising : introducing into a cell a chimeric molecule, a so-called << targeted effector >>, comprising a recognition moiety R having the capacity to specifically interact, within the cell, with a site on an intracellular target molecule T, R interacting with T with a first affinity A1 and an effector moiety, E, covalently linked to said recognition moiety R, E being a molecule, or a portion thereof, which has an initial capacity to exert an effect on at least one molecule M, and which when it is covalently linked to R, acquires the capacity to specifically exert the effect on the intracellular target molecule T, wherein the targeted effector interacts with T with a second affinity A2, the affinity A1 or the affinity A2 corresponding to a Kd of less than 1 x 10<-8> M, and the properties of T and / or of C are specifically modulated by the effector moiety E.</p> |