Methods and apparatus for successively ligating double-stranded DNA molecules on a solid phase

摘要

An object of this invention is to provide a method that enables successive ligation of many DNA molecules while specifying the ligation orientation without cloning, to produce a DNA molecule in which many DNA molecules are ligated by using this method, and to provide a use for the DNA molecule obtained by using this method. Amethod to ligate many DNA molecules was developed by utilizing a solid phase for ligating DNA molecules that enables successive ligation of many DNA molecules without cloning like that in the liquid phase. In addition, the method allowed specification of the ligation orientation of the DNA molecules, by using non-palindromic sequences at the protruding ends of the DNA molecules to be ligated. <IMAGE>