PRODUCTION OF HETEROLOGOUS PROTEINS

摘要

The invention provides a vector for expressing a heterologous gene encoding a polypeptide of interest in a Gram-negative prokaryote and targeting the expressed polypeptide to the outer membrane and/or periplasmic space thereof, the vector comprising nucleic acid encoding a stromal targeting domain (STD). Also provided are host cells containing the vectors, compositions containing the host cells, and the uses of the compositions and host cells.