发明名称 |
Fluorescence polarization-based homogeneous assay for deoxynivalenol determination in grains |
摘要 |
A homogeneous assay for determining the deoxynivalenol (DON) content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with water. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to DON. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating DON to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The DON concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of DON solutions of known concentration.
|
申请公布号 |
US2002055180(A1) |
申请公布日期 |
2002.05.09 |
申请号 |
US20010903061 |
申请日期 |
2001.07.11 |
申请人 |
NASIR MOHAMMAD SARWAR;JOLLEY MICHAEL E. |
发明人 |
NASIR MOHAMMAD SARWAR;JOLLEY MICHAEL E. |
分类号 |
G01N33/542;G01N33/569;(IPC1-7):G01N21/76 |
主分类号 |
G01N33/542 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|