| 摘要 |
A drain joint of a pump block is opened, a piston (19a) is pushed while a piston (13a) is fixed, for charging a passage between a Luer-Lok joint (17) and an intersection part as well as a passage between the intersection part and the drain joint with a buffer. Thereafter the piston (13a) is pushed while the piston (19a) is fixed, to charge a passage (7a) with a polymer. Then, the drain joint is closed, the piston (13a) is pushed and the piston (19a) is pulled in response to the amount of pushing, for charging the passage between the intersection point and the Luer-Lok joint (17) with the polymer. Thereafter, the piston (13a) is pushed while the piston (19a) is fixed, to charge a capillary column with the polymer.
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