NUCLEIC ACID DETECTING METHOD WITH IMPROVED HYBRIDIZATION EFFICIENCY OF PCR PRODUCT-PROBE

摘要

PURPOSE: A nucleic acid detecting method with improved hybridization efficiency of PCR product-probe is provided, thereby inhibiting PCR products from binding together and increasing hybridization efficiency between PCR product and probe. Therefore, the detection of nucleic acid can be more correct. CONSTITUTION: The nucleic acid detecting method using improved hybridization efficiency of PCR product-probe comprises the steps of: inserting nucleic acid sample into a vessel containing a PCR solution comprising any one of sense and anti-sense primer fixed magnetic bead and a fluorescence-labeled primer; amplifying the nucleic acid sample at PCR temperature; heat denaturing the amplified double strand PCR product into single strand PCR product; fixing nucleic acid synthesized by a primer which is fixed on the magnetic bead on the inner side of the vessel using magnetic power; hybridizing the remaining PCR solution with a support having a probe capable of specifically binding with a target protein; removing and washing the unbound PCR product and solution; and determining the fluorescence level of PCR product-probe hybridization on the support.