| 摘要 |
PURPOSE: A method for measuring cholesterol concentration and measuring kit for the same is provided to achieve improved accuracy of analysis result and reduce time and costs for analysis. CONSTITUTION: A method comprises a first step of mixing the blood sample with a solution, and removing blood cell by passing the mixture through a filter; a second step of injecting the blood sample into a pipe which is branched off to four capillary pipes; a third step of shifting the blood to the capillary pipes with a non-treated membrane, a membrane which is treated with an antibody with respect to a low concentration lipoprotein, a membrane which is treated with an antibody with respect to a high concentration lipoprotein, and a membrane treated with both antibodies, such that only the target lipoprotein passes through the capillary pipe by the immune reaction with antibody; a fourth step of shifting blood cell samples passed through the capillary pipe, to a membrane treated with a surface active agent, and permitting cholesterol component to be dissolved from the lipoprotein in the sample; a fifth step of shifting the solution obtained in the fourth step to membranes of each capillary pipe treated with cholesterol decomposition enzyme and color developing agent so as to permit the color developing agent to be dissolved and cholesterol to react with the enzyme; a sixth step of shifting the reaction solution to the reaction space where the enzyme for the color developing agent is fixed; and a seventh step of checking cholesterol concentration for each capillary pipe by measuring the degree of color development.
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