摘要 |
PURPOSE: Provided is an introduction of CMS to a leaf mustard with a symmetric multi-cell fusion process based which supplements an asymmetric fusion process to obtain CMS objects so that it helps to develop inbred line with high fertility. CONSTITUTION: The introduction of CMS to the leaf mustard with the symmetric multi-cell fusion process based is characterized by fusing cabbages, broccolis and the leaf mustards by cell unit in a symmetric way to produce callus and differentiating again to transplant to a pot wherein the three fused materials are mixed to make one fused cell and CMS objects with various forms and the callus is differentiated again at 3% MS medium with 5mg/l of zeatin and 2mg/l of IAA added as a plant growth adjuster.
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