CELL LINE COMPRISING THE PROMOTER OF CYCLOOXYGENASE-2 (COX-2) AND A WITNESS GENE, AND USE THEREOF IN THE SEARCH OF COX-2 TRANSCRIPTIONAL INDUCTION SELECTIVE INHIBITORS

摘要

The cell lines comprises a DNA construct that comprises all or part of a promoter sequence of the gene coding for cyclooxygenase 2 (cox-2) and a reporter gene, operatively joined to each other, such that said promoter sequence of the cox-2 gene controls the expression of said reporter gene in response to a suitable stimulus. The assay method comprises bringing said cell line into contact with the compound to be assayed and determining the existence of a signal indicative of the expression of activity due to the reporter gene. This method is suitable for the induction at a transcriptional level of cox-2 by suitable stimuli. <IMAGE>