发明名称 |
METHOD OF DETERMINING PRION STRAIN |
摘要 |
The present invention provides assays for identifying the levels of both protease sensitie and protease resistant conformers of PrPSc in a sample. In a preferred embodiment, the assay comprises determining levels of total PrPSc in a sample, subjecting the PrPSc fraction to treatment with a protease that selectively hydrolyzes the protease snesitive PrPSc (sPrPSc) conformers, and quantifying the elvels of sPrPSc in the sample. The ability to detect sPrPSc allows early detection of prions, since the PrPSc in easily accessible biological samples such as blood is predominantly sPrPSc. The ratio of sPrPS c to rPrpSc also allows the identification of a particular prion strain in an infected sample.
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申请公布号 |
CA2426062(A1) |
申请公布日期 |
2002.05.02 |
申请号 |
CA20012426062 |
申请日期 |
2001.09.21 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
PRUSINER, STANLEY B.;SAFAR, JIRI |
分类号 |
A01N1/02;C12N11/00;G01N33/53;G01N33/531;G01N33/566;G01N33/68;(IPC1-7):A61K38/00;A61K39/00;A61K49/00;C08G69/26;A61K39/38;A61K39/395;A61K39/42;G01N33/567 |
主分类号 |
A01N1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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