| 摘要 |
A method of gene amplification which comprises annealing primers to a target gene of an animal, a plant, etc. and then amplifying the gene by using a thermotolerant nucleic acid synthase such as Taq polymerase or Pfu polymerase, characterized by using at least one high-pressure fluid selected from the group consisting of supercritical fluids such as carbon dioxide, subcritical fluids, high-pressure gases and liquids as the reaction medium. It is preferable to amplify the gene by using a surfactant-coated enzyme obtained by coating a thermotolerant nucleic acid synthase with a surfactant and controlling the reaction temperature.
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