| 摘要 |
The invention concerns a method for the multiple fluorescence detection of fluorophores. The task assignment of the invention, to develop a category-related method with which the described disadvantages of the state of the art are avoided and with which an ultrasensitive and quick detection of multiple fluorescences of fluorophores in the range of sub-nanoseconds to a few milliseconds is simultaneously ensured, is solved by a simultaneous measurement of the decay time of the fluorescences, where the excitation wave lengths for the individual fluorophores, delayed through an optical delay 4 in the range of sub-nanoseconds to some milliseconds, are conducted to the objects of examination so that the fluorescences can be excitated and detected one after the other. For the differentiation between at least two fluorophores in addition to their spectral characteristics, the decay behaviour of the fluorescence processes is examined by the displacement of electronic gates in the nanosecond range along a timing axis.
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