METHOD FOR INTRODUCING ANTISENSE OLIGONUCLEOTIDES INTO EUCARYOTIC CELLS

摘要

The present invention relates to a method for introducing one or more antisense oligonucleotides into one or more eucaryotic cells using one or mo re lipid formulations comprising one or more cationic lipids of Formula I and optionally at least one neutral lipid. In particular, the present invention relates to a method for introducing one or more antisense oligonucleotides into one or more eucaryotic cells using a lipid formulation comprising dimethyldioctadecylammonium bromide (DDAB) and at least one neutral lipid, especially dioleylphosphatidylethanolamine (DOPE). The invention also relate s to kits for carrying out the invention, compositions for carrying out the invention, and compositions formed while carrying out the invention. Further , the present invention relates to a method for inhibiting or preventing cell growth or proliferation, and a method for inhibiting or preventing expressio n of one or more proteins.