| 摘要 |
<p>The present invention relates to a method for selecting a functional nucleic acid molecule functioning in a cell, cytoplasm or nucleus, which comprises constructing an expression vector which comprises, under the control of a promoter, a candidate nucleic acid sequence comprising a randomized sequence in a portion of a nucleic acid molecule or in another nucleic acid ligated to the nucleic acid molecule; introducing the expression vector into a cell; culturing the cell to express the nucleic acid molecule; collecting and destroying the cell to prepare an extract of an entire cell, a cytoplasm fraction or a nucleus fraction; incubating the extract of an entire cell, the cytoplasm fraction or the nucleus fraction for a certain period of time; and obtaining a nucleic acid molecule remaining in the extract of an entire cell, the cytoplasm fraction or the nucleus fraction, and to a novel functional nucleic acid molecule selected by this method.</p> |
