发明名称 |
IMMOBILIZATION OF BIOPOLYMERS TO AMINATED SUBSTRATES BY DIRECT ADSORPTION |
摘要 |
An assay article for detection biopolymers contained in a sample is described. The assay article includes a substrate and a biopolymer directly adsorbed on the surface of the substrate. A plurality of biopolymers may be adsorbed on the surface of the substrate to form an array. Also disclosed is a method of making the assay article. In the preferred method, an aminated polypropylene substrate is used. A biopolymer is contacted with the aminated substrate under a condition sufficient for direct adsorption of the biopolymer on the surface of the substrate. A method of detecting a target biopolymer contained in a sample is also disclosed. In this method, a substrate is contacted with either a probe or target biopolymer under a condition sufficient for a direct adsorption of either the probe or target biopolymer on the substrate to form a probe assay article or a target assay article. Then, the probe assay article is contacted with the target biopolymer, or the target assay article is contacted with the probe biopolymer under a condition that allows the formation of a probe-target complex. Finally, the complex is detected and the presence of the complex is used as a measurement for the presence or the amount of the biopolymer target contained in the sample. |
申请公布号 |
WO0234950(A2) |
申请公布日期 |
2002.05.02 |
申请号 |
WO2001US43046 |
申请日期 |
2001.10.22 |
申请人 |
BECKMAN COULTER, INC. |
发明人 |
RAMPAL, JANG, B.;MATSON, ROBERT, S. |
分类号 |
G01N33/53;C07B61/00;C07H21/00;C12M1/00;C12N15/09;C12Q1/68;C40B40/06;C40B40/10;C40B60/14;G01N33/543;G01N33/545;G01N37/00 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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