发明名称 |
A COMBINED ELECTROPORATION AND MICROINJECTION METHOD FOR THE PENETRATION OF LIPID BILAYER MEMBRANES |
摘要 |
Disclosed is a method for penetration of lipid bilayer membranes in order to insert at the tip of a hollow needle-shaped object, such as a micropipet-, into a container formed of a lipid bilayer membrane, wherein said container is placed between said needle-shaped object, with the tip of said needle-shaped object placed in contact with said conainer in such a way that it applies a mechanical force to the lipid membrane of said container, thus mechanically straining it, and a second electrode, whereupon a transient electric pulse of 1-to-10<3> V/cm is applied between the electrodes, resulting in a focused electrical field over said container which induces a dielectric breakdown of the lipid bilayer causing the needle-shaped object to penetrate the container. Disclosed is also an electroinjection method based on the above method, wherein substances are introduced through the needle-shaped object and into the container after penetration of the needle-shaped object.
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申请公布号 |
WO0233066(A1) |
申请公布日期 |
2002.04.25 |
申请号 |
WO2001SE02301 |
申请日期 |
2001.10.19 |
申请人 |
CELLECTRICON AB;ORWAR, OWE;KARLSSON, MATTIAS;CHIU, DANIEL |
发明人 |
ORWAR, OWE;KARLSSON, MATTIAS;CHIU, DANIEL |
分类号 |
C12N15/09;B01L3/02;C12N13/00;C12N15/89;(IPC1-7):C12N13/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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