Reverse sample genome probing

摘要

A reverse genome probe process for detecting the presence of selected microorganisms (which may be bacteria, fungi or viruses) consists of:(a) affixing a nucleic acid preparation from each of the selected microorganisms to a solid surface;(b) preparing a "reverse" probe by labelling a nucleic acid preparation obtained from the sample;(c) contacting the reverse probe with the solid surface under conditions which permit hybridization; and(d) detecting the presence of any reverse genome probe.By using the "reverse" probe (i.e., a probe prepared from the sample, as opposed to a probe prepared from a selected microorganism), a single incubation may be used for the initial characterization of an unknown sample.