| 摘要 |
A method and device are provided for simultaneously creating a plurality of identical micro-arrays of biological samples. The invention utilizes a plurality of substrates, each of which having a top side, a bottom side, and a pattern of through-holes. Each through-hole has a wider upper cross-section, a narrower lower cross-section, and a step or plateau formed in the transition area. When the substrates are stacked, through-holes are in registry and for m tunnels extending through the stack of substrates. Reagents of interest are caused to flow through the tunnels and deposit on the step or plateau area. A barrier layer may be provided to prevent leak-through between neighboring holes. After the desired reagents have been deposited, the substrates are separated. In this manner a series of micro-arrays, each capable of containi ng hundreds or thousands of biological samples such as cDNA fragments, is forme d simultaneously.
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