PARAMYXOVIRUS VECTOR FOR TRANSFERRING FOREIGN GENE INTO SKELETAL MUSCLE

摘要

It is examined whether or not a recombinant Sendai virus (SeV) vector is usable in transporting a gene into a skeletal muscle by using LacZ reporter gene and insulin-like growth factor gene. As a result, the transgene expression is sustained for 1 month at the longest after the injection. Compared with a control, the transfer of the insulin-like growth factor gene causes a significant increase in regenerated fibers and a significant increase in slipped muscle fibers, i.e., an indication of enlargement. Also, the total muscle fiber number is increased thereby. Thus, it is indicated that the paramyxovirus vector containing Sendai virus achieves the high-level expression of the transgene in the skeletal muscle and that the transfer of the insulin-like growth factor gene with the use of the paramyxovirus vector is seemingly highly useful in treating neuromuscular disorders.