摘要 |
This invention provides cyanobacteria as an alternative source of <i>ahas</i> and <i>pds</i> genes for plant transformations and for selectable marking. In particular, it provides for cyanobacteria, for example, <i>Synechocystis</i>, as a source of genes encoding herbicide insensitive proteins, and elements of genes for control of expression in plastids. Nucleic acid fragments, both the acetolactate synthase (<i>ahas</i>) large subunit and the <i>ahas</i> small subunit, were found to provide herbicide resistance. Also, the present invention provides novel <i>Synechocystis</i> mutant phytoene desaturase (<i>PDS</i>) gene conferring resistance to 4'-fluoro-6-[(alpha,alpha,alpha,-trifluoro-m-tolyl)oxy]-picolinamide, a bleaching herbicide. The present invention provides improvements to method involving cyanobacteria for the screening of compounds, including a new high-through-put protocol that is a rapid and cost effective way to identify target site genes. |
申请人 |
AMERICAN CYANAMID COMPANY;KAKEFUDA, GENICHI;KOOP, HANS-ULRICH;STURNER, STEPHEN;ZHEN, RUI-GUANG |
发明人 |
KAKEFUDA, GENICHI;KOOP, HANS-ULRICH;STURNER, STEPHEN;ZHEN, RUI-GUANG |