发明名称 |
HIGH THROUGHPUT OR CAPILLARY-BASED SCREENING FOR A BIOACTIVITY OR BIOMOLECULE |
摘要 |
Provided is a method of screening or enriching a sample containing polynucleotides from a mixed population of organisms. The method includes creating a DNA library from a plurality of nucleic acid sequences of a mixed population of organisms and separating clones containing a polynucleotide sequence of interest on an analyzer detects a detectable molecule on a probe or bioactive substrate. The analyzer includes FACS devices, SQUID devices an d MSC devices. The separated or enrich library can then be further process by activity based screening or sequence based screening. In addition, the enriched sequence can be compared to a database and to identify sequences in the database which have homology to a clone in the library thereby obtaining a nucleic acid profile of the mixed population of organisms.
|
申请公布号 |
CA2393374(A1) |
申请公布日期 |
2002.04.18 |
申请号 |
CA20012393374 |
申请日期 |
2001.10.10 |
申请人 |
DIVERSA CORPORATION |
发明人 |
KELLER, MARTIN;LAFFERTY, WILLIAM MICHAEL;SHORT, JAY M. |
分类号 |
G01N33/53;B01J19/00;B01L3/00;C12M1/00;C12N1/00;C12N15/09;C12N15/10;C12Q;C12Q1/02;C12Q1/68;G01N21/03;G01N33/542;G01N33/543;G01N37/00;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/53 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|