Delivery of polypeptide-encoding plasmid dna into the cytsol of macrophages by attenuated suicide bacteria

摘要

The invention relates to the introduction of DNA or RNA sequences into a mammalian cell to achieve controlled expression of a polypeptide. It is therefore useful in gene therapy, vaccination, and any therapeutic situation in which a polypeptide should be administered to a host or cells of said host, as well as for the production of polypeptides by mammalian cells, e.g., in Culture or in transgenic animals.