COMPOSITIONS AND METHODS FOR REDUCING OLIGONUCLEOTIDE HYBRIDIZATION AND PRIMING SPECIFICITY

摘要

Annealing promoting compounds (APCs) are used to reduce the hybridization specificity of oligonucleotide duplexes containing one or more nucleotide mismatches as compared to perfectly base-paired oligonucleotide duplexes. These APCs are used in methods for the detection of target oligonucleotides that vary in nucleic acid sequence by one or more nucleic acid as compared to a control sequence.