| 摘要 |
A method and system for the detection of a target nucleic acid in a sample solution. The target nucleic acid comprises a first and a second end sequenc e, one of the end sequences being a 5' end sequence and the other end sequence being a 3' end sequence. The method comprises: (a) attaching to a solid surface a first oligonucleotide probe, at least a portion of which is complementary to the first end sequence of the target nucleic acid; (b) contacting the solid surface with the sample solution, thereby allowing the first probe to bind the target nucleic acid; (c) providing a second semiconductor nanoparticle to which has been attached a second oligonucleoti de probe, at least a portion of which is complementary to the second end sequen ce of the target nucleic acid; (d) contacting the solid surface of step (b) wit h the second nanoparticle, thereby allowing the second probe to bind the bound target nucleic acid; (e) providing a first semiconductor nanoparticle to whi ch has been attached the first oligonucleotide probe and pre-incubating the fir st nanoparticle with the target nucleic acid, thereby allowing the first probe to bind the target nucleic acid; (f) contacting the solid surface of step (d) with the pre-incubated first nanoparticle, thereby allowing the target nucle ic acid bound to thefirst probe to bind the second probe on the second nanoparticle; and (g) detecting the presence of the nanoparticles on the sol id surface, thereby detecting the target nucleic acid.
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