CASEIN PEPTIDE FRAGMENTS WITH GROWTH-INFLUENCING ACTIVITY ON CELL CULTURES

摘要

A decapeptide was isolated from an enzymatic hydrolysis of bovine casein, the sequence of which could be clearly identified in the primary sequence of casein. Said decapeptide, also produced by synthetic chemical means, influences the growth of the insect cell line IPLB-Sf-21, in a concentration-dependent manner. A tripeptide which appears twice in the sequence of the decapeptide and of casein has a yet stronger effect than the decapeptide, which is also concentration-dependent. Said peptide can thus be used to advantage as an adjunct for serum-free, chemically defined cell culture media.