发明名称 |
Method of preparing normalized and/or subtracted cDNA |
摘要 |
<p>A method of preparing normalized and/or subtracted cDNA; a method in which the cDNA that is normalized and/or subtracted is in the form of uncloned cDNA (cDNA tester); a method of preparing normalized and/or subtracted cDNA comprising the steps of: (a> preparing cDNA tester; (b) preparing normalization and/or subtraction RNA driver; (c) conducting normalization and/or subtraction in two steps in any order, or conducting normalization/subtraction as a single step and mixing the normalization/subtraction RNA driver with said cDNA tester; (d) adding an enzyme capable of cleaving single strand sites on RNA drivers nonspecifically bound to cDNA tester; (e) removing said single strand RNA driver cleaved in step d) from the tester and removing tester/driver hybrids; and (f) recovering the normalized and/or subtracted cDNA; and a method of efficiently preparing normalized and/or subtracted long-chain, full-coding, and full-length cDNA libraries are provided.</p> |
申请公布号 |
EP1197552(A2) |
申请公布日期 |
2002.04.17 |
申请号 |
EP20010120108 |
申请日期 |
2001.08.22 |
申请人 |
RIKEN;HAYASHIZAKI, YOSHIHIDE |
发明人 |
HAYASHIZAKI, YOSHIHIDE |
分类号 |
C12N15/10;(IPC1-7):C12N15/10;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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