| 摘要 |
PROBLEM TO BE SOLVED: To obtain an alkaline pullulanase that acts at an alkaline region and shows and adequate enzymatic activity even in the presence of a surfactant and a gene encoding the enzyme as well as a method for efficiently mass-producing the alkaline pullulanase by the genetic engineering technique. SOLUTION: The objective alkaline pullulanase is found in enzymes produced by microorganisms in the soil, and a gene encoding the enzyme is cloned. The enzyme has a specific amino acid sequence derived from a bacterium belonging to the genus Bacillus, or has an amino acid sequence modified by deleting, substituting or adding one amino acid or more from, in or to the amino acid sequence. A gene encoding the enzyme, a recombinant vector containing the gene, a transformant containing the recombinant vector, and a method for producing the enzyme using these are also provided. |
