发明名称 |
Gj¶rmutanter med N-glykosyleringsdefekter, fremgangsmåte for fremstilling av gj¶rmutanter, og anvendelse av disse, samtfremgangsmåte for fremstilling av et rekombinant protein |
摘要 |
Saccharomyces mutants with defects in N-glycosylation which are obtainable by [<3>H]-mannose suicide selection, introduction of one or more selective markers, selection of those strains which, after transformation with the plasmid YEpL/GOD, on cultivation under standard conditions secrete 10 mg/l GOD or more into the culture medium, are allelic to the ngd mutations contained in Saccharomyces cerevisiae DSM 7042, DSM 7338, DSM 7160 and/or 7340, and express proteins with a uniform carbohydrate structure. |
申请公布号 |
NO312246(B1) |
申请公布日期 |
2002.04.15 |
申请号 |
NO19930002811 |
申请日期 |
1993.08.06 |
申请人 |
ROCHE DIAGNOSTICS GMBH |
发明人 |
LEHLE, LUDWIG;LEHNERT, KLAUS;KOPETZKI, ERHARD |
分类号 |
C07H21/04;C07K14/395;C12N1/16;C12N1/19;C12N9/04;C12N15/00;C12N15/09;C12P21/00;C12P21/02;C12R1/865 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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