| 摘要 |
The instant invention relates to a method and an apparatus useful for purifying DNA sequencing reaction products. Briefly, a gel filtration medium is combined with a molecular cutoff filter in a single apparatus to isolate DNA sequencing fragments from the sequencing template, enzyme, salt and nucleotides. A preferred embodiment of the instant apparatus depicted in FIG. 1 shows a cylindrical housing having openings at the top and bottom of the housing. The housing may take forms other than cylindrical, e.g., rectangular, octagonal, etc. The apparatus may be used in conjunction with pressure and/or centrifugation to achieve the separation, and the addition of a detergent composition (nonionic, ionic, or zwitterionic), including a bile salt, may also be used.
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