发明名称 REVERSE DETECTION FOR IDENTIFICATION AND/OR QUANTIFICATION OF NUCLEOTIDE TARGET SEQUENCES ON BIOCHIPS
摘要 The present invention relates to a method for the identification and/or the quantification of one or more target nucleotide sequences (3) present homologous to each other and being possibly present in a sample and discrimination from possibly homologous sequences, by using two sets of nucleotide sequences (1 and 2), wherein a first set of nucleotide sequences possibly labelled (1) bind specifically to said target nucleotide sequences (3) in a first step and are detected and/or quantified through hybridisation with a second set of capture nucleotide sequences (2) having at least a part of sequence complementary to the possibly labelled nucleotide sequence (1) of the first set of nucleotide sequences, said capture nucleotide sequence (2) being immobilised upon the surface (4) of a solid support according to an array of at least 4 discrete regions/cm<2>, each of said discrete regions being bonded with one species of capture nucleotide sequences (2), and wherein the identification and quantification of the binding between the possibly labelled nucleotide sequence (1) and their corresponding capture nucleotide sequence (2) is correlated with the identification and the quantification of a target nucleotide sequence (3) present in the sample.
申请公布号 WO0196592(A3) 申请公布日期 2002.04.11
申请号 WO2001BE00101 申请日期 2001.06.14
申请人 FACULTES UNIVERSITAIRES NOTRE-DAME DE LA PAIX;REMACLE, JOSE;ART, MURIEL;LOCKMAN, LAURENCE;ZAMMATTEO, NATHALIE 发明人 REMACLE, JOSE;ART, MURIEL;LOCKMAN, LAURENCE;ZAMMATTEO, NATHALIE
分类号 C12M1/00;C12Q1/68 主分类号 C12M1/00
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