| 摘要 |
The present invention discloses nucleic acid detector probes for specific detection and/or quantification of target nucleic acid sequences and detecti on and/or quantification methods using these probes. In the absence of target nucleic acid sequence, a first oligonucleotide and a third oligonucleotide a re bound to each other in a conformation which brings two member of an interacting moiety pair (labels) into close spatial proximity. Cooperative binding of the first oligonucleotide and a second oligonucleotide to a targe t nucleic acid sequence causes displacement of the third oligonucleotide from the first oligonucleotide probe resulting in separation of the two members o f the interacting moiety pair (labels). The spatial separation of the moieties (labels) is detectable, and indicates the presence and/or amount of the targ et nucleic acid sequence. The method is useful for detection and/or quantification of a specific nucleic acid sequence as well as the detection of sequence alteration in the target nucleic acid sequence.
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