| 摘要 |
A method for producing a DNA microarray for biological assays comprising a plurality of biosites located on a solid substrate. Each biosite has at least one set of chemically presynthesized, oligonucleotides of medium to long lengths attached to the substrate by predominantly non-covalent means, such as electrostatic, hydrophobic, hydrogen bonding, van der waal forces, etc. The oligonucleotides exhibit no predetermined spatial orientation on the substrate surface. The oligonucleotides are chemically unmodified having a length of about 30 bases or longer. Preferably, the oligonucleotides are from about 45 bases to 85 bases, but some embodiments may include very long oligonucleotides of over 100 bases.
|
