The present invention provides methods of producing protein in a recombinant expression system that comprises translation of mRNA transcribed from a heterologous DNA sequence in the expression system, said method comprising the steps of predicting the secondary structure of mRNA transcribed from a native heterologous DNA sequence wherein mRNA transcribed from the modified heterologous DNA sequence has a secondary structure having increased free energy compared to that of the secondary structure of the mRNA transcribed from the native heterologous DNA sequence; and using the modified heterologous DNA sequence in the recombinant expression system for protein production. The invention also provides injectable pharmaceutical compositions comprising a nucleic acid molecule that includes a modified coding sequence. The invention also provides coding sequence.
申请公布号
WO0229088(A2)
申请公布日期
2002.04.11
申请号
WO2001US31451
申请日期
2001.10.04
申请人
THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA;WEINER, DAVID, B.;YANG, JOO-SUNG