The invention describes virus-based amplification vectors for plants containing additional plant-specific internal ribosome entry site (IRES) element(s) allowing for a polycistronic translation and a cap-independent translation of : a) heterologous gene(s); b) whole viral genome or c) viral subgenomic RNAs. Said IRES elements are of plant viral origin, or they are isolated from other organisms or engineered using different synthesis procedures. Said IRES element(s) and said heterologous gene(s) are inserted into ampification vectors and allow for the expression of said heterologous gene(s) in the absence of additional viral promoters, in particular, said expression is achieved through cap-independent translation.
申请公布号
WO0229068(A2)
申请公布日期
2002.04.11
申请号
WO2001EP11629
申请日期
2001.10.08
申请人
ICON GENETICS AG;GLEBA, YURI;DOROKOV, YURII;IVANOV, PETER;ATABEKOV, JOSEPH
发明人
GLEBA, YURI;DOROKOV, YURII;IVANOV, PETER;ATABEKOV, JOSEPH