SYSTEM FOR BENZODIAZEPINE DETECTION

摘要

Biological samples are analyzed for benzodiazepines in a single isocratic an alysis using a chromatographic column system containing an immobilized enzyme reactor which cleaves glucuronic acid-conjugated benzo diazepines, an anion exchange column, a hydrophobic cation exchange column and a reverse-phase analytical column. Preferred meth ods of performing the analysis further involve the use o f a hydrophobic cation exchange precolumn prior to the anion exchange column. The system readily lends itself to automation, automati c periodic sampling and benzodiazepine identification and quantification. The system is particularly well adapted to the determination and identification of benzodiazepines in urine samples.