METHOD OF QUANTIFYING HEMOGLOBIN AND METHOD OF MEASURING GLYCATION RATIO OF HEMOGLOBIN

摘要

A method of quantifying hemoglobin whereby the Hb content in a sample can be easily and accurately determined without causing any environmental problem. Hemoglobin in a sample is denatured with a tetrazolium compound and then the optical change of the sample is measured at an absorption wavelength specific to the denatured hemoglobin thus obtained. The hemoglobin content in the sample can be calculated based on the optical change. The measurement wavelength as described above preferably falls within a range of from 520 to 670 nm. According to this method, the Hb content can be determined at a high accuracy, as shown in fig. 1.