METHOD AND DEVICE FOR MAKING A SUPPORT BEARING A PLURALITY OF DIFFERENT POLYNUCLEOTIDE AND/OR PEPTIDE SEQUENCES

摘要

The invention concerns the manufacture of electronic micro-sensors (chips) for analysing nucleotide and/or peptide sequences. The object is to fix at the surface of said chips a very large number of various probe sequences easily and economically. The method aims at making arrays of sequence probes on a support by anchoring and/or local chemical synthesis in the presence of volatile solvents. Said method is characterised in that it consists in using a support whereon is produced a grid of reaction wells made of light-cured (epoxy) resin; in placing the support in a reaction chamber which is saturated with vapours of the synthesis solvent (acetonitrile); in finally producing the anchorage and optionally the localised chemical synthesis in situ of the probe sequences in the wells using a localised supply of liquids/reagents in each well corresponding to a specific probe-sequence, and in supplying collectively the wells with common liquids/reagents and in ensuring that prior to each localised supply operation the liquid contained in each well is eliminated. The invention also concerns a device for implementing said method.