| 摘要 |
PURPOSE: A duffy blood type binding surface protein(DBP) gene of plasmodium viviax and a detection method of malaria using the same are provided, thereby rapidly and exactly diagnosing the infection of plasmodium vivax. CONSTITUTION: The DBP gene of plasmodium vivax has the nucleotide sequence of SEQ ID NO: 1. The DBP of plasmodium vivax has the amino acid sequence of SEQ ID NO: 2. A recombinant vector KPV DBP-1 containing the DBP gene of plasmodium vivax is produced by inserting the DBP gene of plasmodium vivax into a plasmid pQE30. The transformants are produced by transforming host cells JM109 and M15 with the recombinant vector KPV DBP-1. The method for detecting malaria comprises the steps of: centrifuging the transformants capable of producing the DBP of plasmodium vivax; recovering the supernatant; subjecting the supernatant to a column chromatography; reacting the purified DBP with bovine serum albumin; reacting the resulting solution with a conjugation solution of the goat anti-human IgG and horse radish peroxidase; adding TMB into a resulting solution; and measuring its absorbency. |
