发明名称 |
RECOMBINANT RHABDOVIRUSES AS LIVE-VIRAL VACCINES FOR IMMUNODEFICIENCY VIRUSES |
摘要 |
This invention provides recombinant, replication-competent Rhabdovirus vaccine strain-based expression vectors for expressing heterologous viral antigenic polypeptides such as immunodeficiency virus envelope proteins or subparts thereof. An additional transcription stop/start unit within the Rhabdovirus genome is inserted to express the heterologous antigenic polypeptides. The HIV-1 gp160 protein is stably and functionally expressed, as indicated by fusion of human T cell-lines after infection with the recombinant RVs. Inoculation of mice with the recombinant Rabies viruses expressing HIV-1 gp160 induces a strong humoral response directed against the HIV-1 envelope protein after a single boost with an isolated recombinant HIV-1 gp120 protein. Moreover, high neutralization titers, up to 1:800, against HIV-1 are detected in the mouse sera. These recombinant viral vectors expressing viral antigenic polypeptides provide useful and effective pharmaceutical compositions for the generation of viral-specific immune responses.
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申请公布号 |
WO0155330(A3) |
申请公布日期 |
2002.03.28 |
申请号 |
WO2001US01989 |
申请日期 |
2001.01.22 |
申请人 |
THOMAS JEFFERSON UNIVERSITY;SCHNELL, MATTHIAS, J. |
发明人 |
SCHNELL, MATTHIAS, J. |
分类号 |
C12N15/09;A61K35/76;A61K39/21;A61K48/00;A61P31/18;C07K14/16;C12N7/00;C12N15/86;(IPC1-7):C12P21/06;C12N7/04;A61K39/12;A61K39/205 |
主分类号 |
C12N15/09 |
代理机构 |
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