METHODS AND MEANS OF RNA ANALYSIS

摘要

This invention relates to a method for identifying an accessible region in a test mRNA molecule. In the method a test mRNA molecule is brought into contact with a population of oligonucleotide molecules under conditions where the test mRNA molecule retains its native structure, each of oligonucleotide molecule in the population comprising a random portion, whereby said portion is able to bind a complementary accessible region of an mRNA molecule if present. Binding random oligonucleotides encode sequences complementary to accessible regions. Moreover the invention relates to sequences identified and their use in antisense.