摘要 |
Multifunctional reagent (A) for erythrocytes (RBC) contains: (i) enough of a carbamate (I) to lyse RBC or to cause their spherization, so that they can be determined by a cytometer or automated counter or; (ii) an agent (II) that induces formation of a carbamate, associated with absorption of carbon dioxide by RBC, from carbonate and a nitrogen heterocycle or ammonium ion. Independent claims are also included for the following: (1) carbamates of pyrrolidine, morpholine and piperidine; (2) lysis or spherization of RBC in anticoagulant-treated whole blood by treating with (1); (3) preparing leucocytes (WBC) involving lysis of RBC with (1). |