发明名称 |
CYCLOHEXENE NUCLEIC ACIDS |
摘要 |
Replacement of the furanose moiety of, for example, DNA by a cyclohexene ring gives a new nucleic acid structure: cyclohexene nucleic acids or CeNA. Cyclohexene nucleic acids can be obtained by the classical phosphoramidite chemistry starting from protected cyclohexenyl nucleoside building blocks. Incorporation of cyclohexenyl nucleosides in a DNA chain increases the stability of a DNA/RNA hybrid, the complex formed between cyclohexenyl oligoadenylate and its DNA or RNA complement is of similar stability. CD and NMR studies indicate easy conformational adaptation of a cyclohexenyl nucleoside when incorporated in a natural nucleic acid structure. CeNA is stable against degradation in serum and a CeNA/RNA hybrid is able to activate E. Coli Rnase H, resulting in cleavage of the RNA strand.
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申请公布号 |
WO0149687(A3) |
申请公布日期 |
2002.03.28 |
申请号 |
WO2000IB02041 |
申请日期 |
2000.12.27 |
申请人 |
K. U. LEUVEN RESEARCH & DEVELOPMENT;WANG, JING;HERDEWIJN, PIET |
发明人 |
WANG, JING;HERDEWIJN, PIET |
分类号 |
G01N33/68;A61K9/08;A61K9/14;A61K31/712;A61P25/00;A61P43/00;C07D473/34;C07H19/16;C07H19/20;C07H21/00;C07H21/02;C07H21/04;C12N15/09;(IPC1-7):C07D473/34;A61K31/70 |
主分类号 |
G01N33/68 |
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