发明名称 |
New menE gene of coryneform bacteria, useful when suppressed for increasing fermentative production of L-amino acids, encodes an O-succinylbenzoic acid CoA-ligase |
摘要 |
Isolated polynucleotide (I) from coryneform bacteria that represents the menE gene is: (i) 70% identical with a polynucleotide that encodes a 376 amino acid sequence S2, reproduced; (ii) encodes a polypeptide 70% identical with S2; (iii) a complement of (i) or (ii), or (iv) consisting of 15 consecutive nucleotides from (i)-(iii). Encoded polypeptides preferably have the activity of an O-succinylbenzoic acid CoA-ligase. Independent claims are also included for the following: (1) an integration vector pCR2.1menEint (map reproduced); (2) coryneform bacteria in which activity of the menE gene has been reduced, especially switched off, by deletion; (3) fermentative production of L-aa, especially L-lysine, by growing bacteria of (1); and (4) coryneform bacteria containing a vector that carries a fragment, of at least 15 consecutive nucleotides (nt), from (I). |
申请公布号 |
DE10112106(A1) |
申请公布日期 |
2002.03.28 |
申请号 |
DE2001112106 |
申请日期 |
2001.03.14 |
申请人 |
DEGUSSA AG |
发明人 |
FARWICK, MIKE;HUTHMACHER, KLAUS;PFEFFERLE, WALTER;MARX, ACHIM |
分类号 |
C12N1/21;C12N9/00;C12P13/08 |
主分类号 |
C12N1/21 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|