| 摘要 |
The present invention discloses polyketides and the polyketide synthases and ancillary enzymes that are capable of producing such compounds. More particularly, the present invention discloses polynucleotides and polypeptides associated with (i) a novel polyketide synthase linked to a non-ribosomal peptide synthetase involved in the biosynthesis of albicidins, (ii) a novel phosphopantetheinyl transferase for activating enzymes, particularly polyketide synthases and/or non-ribosomal peptide synthetases, associated with the biosynthesis of albicidins, and (iii) a novel methyltransferase for methylating precursors of albicidins and/or intermediates related to albicidin biosynthesis. The present invention also discloses methods of using the aforementioned polynucleotides and polypeptides for activating polyketide synthases and/or non-ribosomal peptide synthetases, for methylating precursors of albicidins or their analogues and/or intermediates involved in the biosynthesis of albicidins or analogues thereof and for enhancing the level and/or functional activity of albicidins or their analogues. Also disclosed are methods of using the polynucleotides and polypeptides of the invention for the biosynthesis of albicidins or their analogues. |
