摘要 |
An imaging system comprises three or more sources for providing exitation radiation of different wavelengths, an objective lens for focusing one or more of the exitation wavelengths onto a sample to produce fluorescent emission, and a mirror, configured as a geometric beamsplitter, disposed in the transmission path of the emission radiation and exitation radiation subsequent to reflection from the sample and collimation by the lens, wherein the mirror is utilized to reflect one of the collimated exitation and emission radiation such that the emission is directed to a detector and the collimated exitation is directed away from the detector.
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