METHOD FOR GENERATING RECOMBINANT POLYNUCLEOTIDES

摘要

A novel method for producing recombinant polynucleotides in vitro is provided. This method entails the treatment of heteroduplex DNA sequences with a nuclease (preferably, DNase I) and a polymerase (preferably, DNA polymerase I), the enzymes primarily involved in nick translation. The results achieved using this process are superior to that achieved by previous in vivo recombination efforts utilizing specific DNA repair systems.