发明名称 |
Immunoassay for measuring human C-peptide and kit therefor |
摘要 |
<p>An immunoassay for selectively measuring human C-peptide as well as a kit therefor is disclosed. In the method, human C-peptide contained in a sample, a first anti-human C-peptide antibody, and a second anti-human C-peptide antibody which is immobilized on a solid support are reacted to form an immune complex among these three components. The formed immune complex is separated from the non-reacted antibodies and sample; and then the separated immune complex is quantified. The first antibody recognizes an epitope existing in the region from 1st to 10th amino acid residue from the N-terminal of the human C-peptide, and the second antibody recognizes an epitope existing in the region from 1st to 16th amino acid residue from the N-terminal of human C-peptide; with the proviso that the first and second antibodies do not recognize the same epitope so that they can simultaneously bind to said human C-peptide. <IMAGE></p> |
申请公布号 |
EP1191337(A2) |
申请公布日期 |
2002.03.27 |
申请号 |
EP20010305049 |
申请日期 |
2001.06.11 |
申请人 |
FUJIREBIO INC. |
发明人 |
KITAJIMA, SACHIKO;KURANO, YOSHIHIRO;NAKATSUBO, KAORU;NISHIZONO, ISAO |
分类号 |
G01N33/74;(IPC1-7):G01N33/68 |
主分类号 |
G01N33/74 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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